Polyphosphate coatings: A promising strategy to overcome the polycation dilemma

It was the aim of this study to develop a zeta potential changing drug delivery system by decorating lipid-based nanocarriers with polycationic cell penetrating peptide (CPP) and subsequently masking these cationic substructures polyphosphates. In order anchor CPP poly-l-lysine (PLL) on surface oily droplets an o/w nanoemulsion, stearic acid covalently attached peptide. The resulting CPP-decorated were coated phytic tripolyphosphate. elimination polyphosphates due cleavage alkaline phosphatase monitored release monophosphate from nanocarriers, change in cellular uptake studies Caco-2 cells. Polyphosphate PLL-decorated exhibited pronounced conversion ?14.1 mV +4.2 case tripolyphosphate ?9.9 ?2.6 nanocarriers. cells polyphosphate 4-fold improved compared control Furthermore, confocal images showed that majority nanodroplets distributed cytoplasm not being internalized into lysosomes. coating seems be promising simple strategy overcome polycation dilemma.